Western blot results can either be the highlight of the day or a scientist's worst nightmare. Unfortunately, it usually turns out to be the latter of the two. After a long hard day of work, nothing can ruin the day more than seeing your western blot results come out blotchy and unreadable.
1. Choose the right membrane for your application
PVDF (polyvinylidene difluoride) and nitrocellulose are usually the most commonly used membranes for Western blot testing. However, they have different properties that you can take advantage of depending on the type of test. Boster Bio featured products can provide the best antibody products online.
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2. Follow the washing and detection steps carefully.
Washing the test properly is an important step to get the best results. Sometimes even the smallest change in this step can mean the difference between a good quality stain and a low-quality stain.
3. Always try to use good quality antibodies
Like the "secret recipes" you find in restaurants, the "secret" usually lies in purchasing the highest quality products. Unfortunately, there are companies that sell antibodies without testing, causing unnecessary stress to customers. It is always important to know how to find a good primary antibody supplier.
4. Make sure you use the correct incubation rate
It is important to always use the correct shaking speed during the incubation period. Antibodies may not bind well if you shake them too quickly and if you do so too slowly will prevent the antibodies from binding evenly.
Always check that your shock level is balanced before continuing with incubation to avoid silly mistakes that would take up your research.